adherent pc 12 cells  (ATCC)

Journal: Scientific Reports

Article Title: Hyperglycemia-induced mitochondrial abnormalities in autonomic neurons via the RAGE axis

doi: 10.1038/s41598-025-10933-y

Figure Lengend Snippet: Detection of RAGE protein in mitochondria-enriched samples from PC12 NGF cells. A-B , Immunoblots and quantification of the level of expression of the neuronal markers MAP-2, neurofilament light chain (NF-L) and tubulin in whole PC12 samples maintained in media without (-NGF) or with NGF added (+ NGF). C , The immunoblots show the levels of RAGE, VDAC and tubulin detected in the cytosol-enriched and mitochondria-enriched samples from PC12 cells transformed by NGF (PC12 NGF ) maintained in either control (CTL) or high glucose (HG) conditions. C-D , The bar graphs show mean ± SEM levels of each protein after normalization to tubulin or VDAC in cytosol-enriched and mitochondria-enriched samples, respectively. Means were statistically compared by the Mann-Whitney U test; (MAP2: t 4 = 2.695; NF-L: t 4 = 3.297); * p < 0.05. ( N = 3 per group in A and C). For clarity, the blots shown in A and C have been cropped, and the background brightness has been adjusted (complete blots available in Supplementary Data file).

Article Snippet: Adherent PC12 cells (ATCC ® CRL-1721.1TM) were cultured in F-12 K growth media (2.5% fetal bovine serum, 15% horse serum; PC12 GM).

Techniques: Western Blot, Expressing, Transformation Assay, Control, MANN-WHITNEY

Journal: Foods

Article Title: Bioinformatics and Deep Learning Approach to Discover Food-Derived Active Ingredients for Alzheimer’s Disease Therapy

doi: 10.3390/foods14010127

Figure Lengend Snippet: Cell viability ( A ) and acetylcholinesterase (AChE) activity ( B ) Nerve growth factor (NGF)-differentiated PC12 cells with lipopolysaccharide-induced inflammation (LPS, 1 μg/mL) were treated with astragalin, dihydromyricetin, coumarin, quercetin, kaempferol, apigenin, and luteolin for 24 h to measure cell viability and 48 h to measure AChE activity. a–e Different letters on the bar indicated significant differences between the groups at p < 0.05.

Article Snippet: The cells of the PC12 adherent cell line (ATCC CRL-1721.1) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma, Aldrich, St. Louis, MO, USA), supplemented with 10% horse serum, 5% fetal bovine serum, and 1% antibiotic mixture containing penicillin–streptomycin, in a humidified atmosphere at 37 °C with 5% CO 2 .

Techniques: Activity Assay

Journal: Foods

Article Title: Bioinformatics and Deep Learning Approach to Discover Food-Derived Active Ingredients for Alzheimer’s Disease Therapy

doi: 10.3390/foods14010127

Figure Lengend Snippet: Lipid peroxidation ( A ) and protein ( B ) and mRNA expression ( C ) of pro-inflammatory cytokines. Nerve growth factor (NGF)-differentiated PC12 cells with lipopolysaccharide-induced inflammation (LPS, 1 μg/mL) were treated with astragalin, dihydromyricetin, coumarin, quercetin, kaempferol, apigenin, and luteolin for 48 h. a–e Different letters on the bar indicated significant differences between the groups at p < 0.05.

Article Snippet: The cells of the PC12 adherent cell line (ATCC CRL-1721.1) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma, Aldrich, St. Louis, MO, USA), supplemented with 10% horse serum, 5% fetal bovine serum, and 1% antibiotic mixture containing penicillin–streptomycin, in a humidified atmosphere at 37 °C with 5% CO 2 .

Techniques: Expressing