Blocking Assay:Article Title: Anti-PACAP antibodies and uses thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs. prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies, nucleic acids and methods of making thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs. prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies and uses thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Methods of treatment with anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Screening method for identifying anti-PACAP antibodies or antibody fragments suitable for use in treating or preventing PACAP-associated photophobia or light aversion
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Treatment of headache, migraine and/or photophobia conditions using humanized anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Binding Assay:Article Title: Anti-PACAP antibodies and uses thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs. prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies, nucleic acids and methods of making thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs. prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies and uses thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Methods of treatment with anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Screening method for identifying anti-PACAP antibodies or antibody fragments suitable for use in treating or preventing PACAP-associated photophobia or light aversion
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Treatment of headache, migraine and/or photophobia conditions using humanized anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Expressing:Article Title: Anti-PACAP antibodies and uses thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs. prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies, nucleic acids and methods of making thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs. prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies and uses thereof
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Methods of treatment with anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, Va.) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, N.Y.) and further incubated for 1 hr at room temperature.
Article Title: Screening method for identifying anti-PACAP antibodies or antibody fragments suitable for use in treating or preventing PACAP-associated photophobia or light aversion
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
Article Title: Treatment of headache, migraine and/or photophobia conditions using humanized anti-PACAP antibodies
Article Snippet: .. To identify antibodies that block PACAP38 binding to PAC1-R-expressing cells, adherent PC-12 cells (ATCC, Manassas, VA) expressing PAC1-R were used in a Europium-based PAC1-R-expressing cells binding assay. .. Antibody solutions were incubated with N-terminal biotinylated PACAP38 at 10× the final concentration (100 nM or 30 nM) for 1 hr, then added to PC-12 cells that were plated 24 hrs prior in black clear bottom 96 well plates (COSTARTM, Corning Incorporated, Corning, NY) and further incubated for 1 hr at room temperature.
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